tag:blogger.com,1999:blog-51846086760422068.post8209482095093505365..comments2024-03-28T08:37:08.964-07:00Comments on Open Flask: Technique primer: Test tube columnsBaranLabhttp://www.blogger.com/profile/01806245988209508968noreply@blogger.comBlogger10125tag:blogger.com,1999:blog-51846086760422068.post-54428513829133069012019-07-18T14:32:39.841-07:002019-07-18T14:32:39.841-07:00Propane also works pretty wellPropane also works pretty wellAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-36042349993319447162019-07-18T10:44:09.543-07:002019-07-18T10:44:09.543-07:00Regular butane for us. Place the tube near the top...Regular butane for us. Place the tube near the top of the flame.BaranLabhttps://www.blogger.com/profile/01806245988209508968noreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-35469468309635542612019-07-04T06:25:42.824-07:002019-07-04T06:25:42.824-07:00Hi, nice post. what kind of lighter did you use? s...Hi, nice post. what kind of lighter did you use? since the butane as lighter gas in our lab failed to melt the glassware.....Simon Tanghttps://www.blogger.com/profile/09818915739205850441noreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-1903184929486582622019-01-31T08:23:04.586-08:002019-01-31T08:23:04.586-08:00Yeah, we use borosilicate (Pyrex) tubes. Part numb...Yeah, we use borosilicate (Pyrex) tubes. Part number: 47729-528 from VWR.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-7374187351666066672019-01-31T07:03:31.228-08:002019-01-31T07:03:31.228-08:00Which brand of test tubes do you use? Cheaper test...Which brand of test tubes do you use? Cheaper test tubes made of limestone glass can not withstand such heating. Is it pyrex?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-76679401868183653142019-01-25T10:19:58.978-08:002019-01-25T10:19:58.978-08:00One modification that I would suggest for pipette ...One modification that I would suggest for pipette columns is to not use a positive pressure of air/nitrogen or what have you, it's too easy to dry out the silica if you're not careful, or cause an alarming buildup of pressure. Instead, put a SubaSeal on the back end (may need a little parafilm to seal), then poke a long needle through to feed solvent from a syringe, which will also create enough positive pressure to run the column at a reasonable rate (which you can easily control with your hand). I am sure this is what a lot of people already do and I learned it from a few people, but for anyone looking to try out pipette columns it is much better and way less hassle. Also in my opinion far superior to prep TLC.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-56008462150342603342019-01-16T12:40:06.749-08:002019-01-16T12:40:06.749-08:00Or you could just use CombiFlash or analogs.Or you could just use CombiFlash or analogs.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-29091791976922698292019-01-15T09:52:00.247-08:002019-01-15T09:52:00.247-08:00We use Acros brand, "Silica gel for chromatog...We use Acros brand, "Silica gel for chromatography, 0.035-0.070 mm, 60A"<br /><br />At other times depending on what's on sale, we have also used the analogous product from Merck KGaA and in our hands they perform the same.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-1511423925222344362019-01-15T01:52:08.193-08:002019-01-15T01:52:08.193-08:00and.....what kind of SiO2 do you use?and.....what kind of SiO2 do you use?Anonymoushttps://www.blogger.com/profile/11920225151527436971noreply@blogger.comtag:blogger.com,1999:blog-51846086760422068.post-54640992951468982392019-01-14T10:48:45.786-08:002019-01-14T10:48:45.786-08:00An extra piece of advice - when pulling the bottom...An extra piece of advice - when pulling the bottom of the test tube with tweezers or tongs, it's best to also heat the end of the tweezer/tong alongside the test tube. When I've done this in the past and haven't heated the tong, the glass cools too quickly upon contact and you can't get a nice pull to make a usable column. Just my two centsAnonymousnoreply@blogger.com